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anti smad7  (Proteintech)


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    Structured Review

    Proteintech anti smad7
    Anti Smad7, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+smad7/SMAD7+Antibody/pm41898415-156-41-57
    Average 95 stars, based on 143 article reviews
    anti smad7 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Exosomal microRNAs of tuberculous pleural effusion orchestrating TGF-β signaling mediate pleural fibrosis
    Article Snippet: Bacillus Calmette-Guerin (BCG) was obtained from Becton, Dickinson and Company (NJ, USA). .. Antibodies used for western blot analysis were as follows: anti-CD63 (Cat#67605-1-Ig, Proteintech), anti-TSG101 (Cat#ab30871, Abcam), anti-GAPDH (Cat#60004-1, Proteintech), anti-COL1A1 (Cat#14695-1-AP, Proteintech), anti-TGF-β1 (Cat#MAB240, R&D Systems), anti-phospho-smad2/3 (p-Smad2/3) (Cat#8828, Cell Signaling Technology), anti-smad2/3 (Cat#8685, Cell Signaling Technology), anti-CD9 (Cat#20597-1-AP, Proteintech), anti-SDCBP (Cat#68096-1-Ig, Proteintech), anti-β-actin (Cat#81115-1-RR, Proteintech), anti-phospho-ERK1/2 (p-ERK1/2) (Cat#8544, Cell Signaling Technology), anti-Fibronectin (FN) (Cat#15613-1-AP, Proteintech), anti-Vimentin (Cat#10366-1-AP, Proteintech), anti-E-cadherin (Cat#20874-1-AP, Proteintech), anti-ACTA2 (Cat#14395-1-AP, Proteintech), anti-CK8 (Cat#17514-1-AP, Proteintech), anti-ELK1 (Cat#27420-1-AP, Proteintech), anti-ITGB6 (Cat#28378-1-AP, Proteintech), anti-SMURF1 (Cat#55175-1-AP, Proteintech), anti-TGFBR1 (Cat#30117-1-AP, Proteintech), anti-SMAD7 (Cat#25840-1-AP, Proteintech), anti-Ubiquitin (Cat# 20326, Cell Signaling Technology), anti-Flag (Cat#M185-3, MBL), HA (Cat#M180-3, MBL). ..

    Article Title: Rhein alleviates renal interstitial fibrosis by inhibiting Smad3 phosphorylation in TGF- β /Smad signalling pathway
    Article Snippet: RNAiso Plus (9108), reverse transcription kit (RR037B), and cDNA amplification kit (RR055A) were purchased from Takara Bio, Inc. (Kusatsu, Japan). .. The primary antibodies were diluted as follows: anti- α -smooth muscle actin (SMA) [1:1 000 for Western blotting (WB) and 1:200 for immunohistochemistry (IHC), ab7817, Abcam, Cambridge, UK], anti-fibronectin (1:1 000 for WB, ab2413, Abcam, Cambridge, UK), anti-collagen I (1:1 000 for WB, ab260043, Abcam, Cambridge, UK), anti-Smad2 (1:500 for WB, 12570-AP, Proteintech, Wuhan, China), anti-p-Smad2 (1:500 for WB, AF8314, Affinity, Liyang, China), anti-Smad3 [1:1 000 for WB, #9523, cell signalling technology (CST), Danvers, USA], anti-p-Smad3 (1:500 for WB, #9520, CST, Danvers, USA), anti-Smad4 (1:500 for WB, 10,231 AP, Proteintech, Wuhan, China), anti-Smad7 (1:500 for WB, 25840-1AP, Proteintech, Wuhan, China), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:1 000 for WB, AF7021, Affinity, Liyang, China). ..

    other:

    Article Title: Exosomal microRNAs of tuberculous pleural effusion orchestrating TGF-β signaling mediate pleural fibrosis.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Proteintech), anti-SMURF1 (Cat#55175-1-AP, Proteintech), anti-TGFBR1 (Cat#30117-1-AP, Proteintech), anti-SMAD7 (Cat#25840-1-AP, Proteintech), anti-Ubiquitin (Cat# 20326, Cell Signaling Technology), antiFlag (Cat#M185-3, MBL), HA (Cat#M180-3, MBL).

    Immunohistochemistry:

    Article Title: Rhein alleviates renal interstitial fibrosis by inhibiting Smad3 phosphorylation in TGF- β /Smad signalling pathway
    Article Snippet: RNAiso Plus (9108), reverse transcription kit (RR037B), and cDNA amplification kit (RR055A) were purchased from Takara Bio, Inc. (Kusatsu, Japan). .. The primary antibodies were diluted as follows: anti- α -smooth muscle actin (SMA) [1:1 000 for Western blotting (WB) and 1:200 for immunohistochemistry (IHC), ab7817, Abcam, Cambridge, UK], anti-fibronectin (1:1 000 for WB, ab2413, Abcam, Cambridge, UK), anti-collagen I (1:1 000 for WB, ab260043, Abcam, Cambridge, UK), anti-Smad2 (1:500 for WB, 12570-AP, Proteintech, Wuhan, China), anti-p-Smad2 (1:500 for WB, AF8314, Affinity, Liyang, China), anti-Smad3 [1:1 000 for WB, #9523, cell signalling technology (CST), Danvers, USA], anti-p-Smad3 (1:500 for WB, #9520, CST, Danvers, USA), anti-Smad4 (1:500 for WB, 10,231 AP, Proteintech, Wuhan, China), anti-Smad7 (1:500 for WB, 25840-1AP, Proteintech, Wuhan, China), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:1 000 for WB, AF7021, Affinity, Liyang, China). ..



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    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    R&D Systems mouse primary antibody against human smad7
    (A) Representative IHC staining images of TGF-β1, p-Smad3, and <t>Smad7</t> in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.
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    Image Search Results


    (A) Representative IHC staining images of TGF-β1, p-Smad3, and Smad7 in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.

    Journal: PeerJ

    Article Title: Dapagliflozin attenuates diabetic renal fibrosis by inhibiting macrophage-myofibroblast transition via the TGF-β1-Smad3/7 pathway

    doi: 10.7717/peerj.21321

    Figure Lengend Snippet: (A) Representative IHC staining images of TGF-β1, p-Smad3, and Smad7 in renal tissues. Diabetic mice showed increased TGF-β1/p-Smad3 and decreased Smad7, which was reversed by DAPA. Scale bar = 2 0 µm. (B) Western blot analysis of TGF-β1, p-Smad3, and Smad7 protein expression in renal tissues (normalized to β-actin). TGF-β1 and p-Smad3/Smad3 ratio were increased and Smad7 was decreased in diabetic mice, and DAPA restored the Smad3/Smad7 balance. Data are presented as mean ± SD with individual data points (dots). n = 6 per group for I HC; n = 3 per group for Western blot. * p < 0.05 vs. Ctrl group; # p < 0.05 vs. DKD group.

    Article Snippet: Membranes were blocked with 5% skim milk (1 h, room temperature), then incubated overnight at 4 °C with primary antibodies: TGF-β1 (A16640, 1:1000, Abclonal, Wuhan, China), p-Smad3 (AP0727, 1:1000, Abclonal, Wuhan, China), Smad3 (A22133, 1:2000, Abclonal, Wuhan, China), Smad7 (A16396, 1:2000, Abclonal, Wuhan, China), α-SMA (A17910, 1:2000, Abclonal, Wuhan, China), Col-I (14695-1-AP, 1:2000, Proteintech, Wuhan, China), and β-actin (db13986, 1:10,000, Diagbio, Hangzhou, China).

    Techniques: Immunohistochemistry, Western Blot, Expressing

    In DKD, hyperglycemia induces renal inflammation and activation of the TGF-β1/Smad3 pathway, which drives macrophages (F4/80 + ) to undergo MMT, acquiring myofibroblast characteristics (α-SMA + ) and producing Col-I, leading to tubulointerstitial fibrosis and progressive kidney dysfunction. DAPA inhibits TGF-β1/Smad3 pathway activity, upregulates Smad7 expression, restores Smad3/Smad7 homeostatic balance, and is highly likely to block the MMT process through this mechanism, thereby reducing α-SMA and Col-I deposition, and ultimately ameliorating diabetic renal fibrosis. Created at BioRender.com.

    Journal: PeerJ

    Article Title: Dapagliflozin attenuates diabetic renal fibrosis by inhibiting macrophage-myofibroblast transition via the TGF-β1-Smad3/7 pathway

    doi: 10.7717/peerj.21321

    Figure Lengend Snippet: In DKD, hyperglycemia induces renal inflammation and activation of the TGF-β1/Smad3 pathway, which drives macrophages (F4/80 + ) to undergo MMT, acquiring myofibroblast characteristics (α-SMA + ) and producing Col-I, leading to tubulointerstitial fibrosis and progressive kidney dysfunction. DAPA inhibits TGF-β1/Smad3 pathway activity, upregulates Smad7 expression, restores Smad3/Smad7 homeostatic balance, and is highly likely to block the MMT process through this mechanism, thereby reducing α-SMA and Col-I deposition, and ultimately ameliorating diabetic renal fibrosis. Created at BioRender.com.

    Article Snippet: Membranes were blocked with 5% skim milk (1 h, room temperature), then incubated overnight at 4 °C with primary antibodies: TGF-β1 (A16640, 1:1000, Abclonal, Wuhan, China), p-Smad3 (AP0727, 1:1000, Abclonal, Wuhan, China), Smad3 (A22133, 1:2000, Abclonal, Wuhan, China), Smad7 (A16396, 1:2000, Abclonal, Wuhan, China), α-SMA (A17910, 1:2000, Abclonal, Wuhan, China), Col-I (14695-1-AP, 1:2000, Proteintech, Wuhan, China), and β-actin (db13986, 1:10,000, Diagbio, Hangzhou, China).

    Techniques: Activation Assay, Activity Assay, Expressing, Blocking Assay